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plasmid fuw dcas9 dnmt3a  (Addgene inc)


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    Structured Review

    Addgene inc plasmid fuw dcas9 dnmt3a
    Plasmid Fuw Dcas9 Dnmt3a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+84476/Fuw-dCas9-Dnmt3a+(Plasmid+%2384476)/pm41013732-72-0-8
    Average 93 stars, based on 18 article reviews
    plasmid fuw dcas9 dnmt3a - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: DNA methylation analysis and editing in single mammalian oocytes
    Article Snippet: For the dCas9-Tet1 inactive form, the T7 promoter was added by PCR using primer m dCas9-Tet1 IF forward and reverse and Addgene plasmid 84479 as template. .. For dCas9-Dnmt3a, the T7 promoter was added by PCR using primer m dCas9-Dnmt3a forward and reverse and Addgene plasmid 84476 as template. .. For dCas9-Dnmt3a inactive form, the T7 promoter was added by PCR using primer dCas9-Dnmt3a IF forward and reverse and Addgene plasmid 84478 as template.

    Article Title: Viable offspring derived from single unfertilized mammalian oocytes
    Article Snippet: .. For dCas9-Dnmt3a, the T7 promoter was added by PCR using primer dCas9-Dnmt3a For and Rev ( SI Appendix , Table S4 ) and Addgene plasmid 84476 as the template. .. For the dCas9-Dnmt3a inactive form, the T7 promoter was added by PCR using primer dCas9-Dnmt3a IF For and Rev ( SI Appendix , Table S4 ) and Addgene plasmid 84478 as the template.

    Plasmid Preparation:

    Article Title: DNA methylation analysis and editing in single mammalian oocytes
    Article Snippet: For the dCas9-Tet1 inactive form, the T7 promoter was added by PCR using primer m dCas9-Tet1 IF forward and reverse and Addgene plasmid 84479 as template. .. For dCas9-Dnmt3a, the T7 promoter was added by PCR using primer m dCas9-Dnmt3a forward and reverse and Addgene plasmid 84476 as template. .. For dCas9-Dnmt3a inactive form, the T7 promoter was added by PCR using primer dCas9-Dnmt3a IF forward and reverse and Addgene plasmid 84478 as template.

    Article Title: Viable offspring derived from single unfertilized mammalian oocytes
    Article Snippet: .. For dCas9-Dnmt3a, the T7 promoter was added by PCR using primer dCas9-Dnmt3a For and Rev ( SI Appendix , Table S4 ) and Addgene plasmid 84476 as the template. .. For the dCas9-Dnmt3a inactive form, the T7 promoter was added by PCR using primer dCas9-Dnmt3a IF For and Rev ( SI Appendix , Table S4 ) and Addgene plasmid 84478 as the template.

    Article Title: A complex epigenome-splicing crosstalk governs epithelial-to-mesenchymal transition in metastasis and brain development.
    Article Snippet: 1Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.. 2Institute of Human Genetics–UMR9002 CNRS-University of Montpellier, Montpellier, France.. 3Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry & Biomedical Science, Queens University Belfast, Belfast, UK.

    other:

    Article Title: DNA methylation analysis and editing in single mammalian oocytes
    Article Snippet: For sgRNA, the T7 promoter was added to specific sgRNAs by PCR using different primers and Addgene plasmid 42230 as template.

    CRISPR:

    Article Title: A complex epigenome-splicing crosstalk governs epithelial-to-mesenchymal transition in metastasis and brain development.
    Article Snippet: 1Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.. 2Institute of Human Genetics–UMR9002 CNRS-University of Montpellier, Montpellier, France.. 3Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry & Biomedical Science, Queens University Belfast, Belfast, UK.

    Clone Assay:

    Article Title: A complex epigenome-splicing crosstalk governs epithelial-to-mesenchymal transition in metastasis and brain development.
    Article Snippet: 1Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.. 2Institute of Human Genetics–UMR9002 CNRS-University of Montpellier, Montpellier, France.. 3Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry & Biomedical Science, Queens University Belfast, Belfast, UK.



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    Image Search Results


    ( A and B ) Macrophage migration assay ( A ) and inflammatory gene expression ( B ) in RAW264.7 macrophages cocultured with 3T3-L1 adipocytes infected with lentivirus expressing scramble sgRNA or S7 sgRNA targeting Esr1 promoter along with dCas9-DNMT3a. n = 3/group, * P < 0.05 vs. Scramble by Student’s t test. Images in A are representative from 3 replicate samples. ( C and D ) Macrophage migration assay ( C ) and inflammatory gene expression ( D ) in RAW264.7 macrophages cocultured with 3T3-L1 adipocytes infected with lentivirus expressing scramble sgRNA or S7 sgRNA targeting Esr1 promoter along with dCas9-TET1. n = 3/group, * P < 0.05 vs. Scramble by Student’s t test. Images in C are representative from 3 replicate samples. All data are expressed as mean ± SEM.

    Journal: JCI Insight

    Article Title: Epigenetic programming of estrogen receptor in adipocytes by high-fat diet regulates obesity-induced inflammation

    doi: 10.1172/jci.insight.173423

    Figure Lengend Snippet: ( A and B ) Macrophage migration assay ( A ) and inflammatory gene expression ( B ) in RAW264.7 macrophages cocultured with 3T3-L1 adipocytes infected with lentivirus expressing scramble sgRNA or S7 sgRNA targeting Esr1 promoter along with dCas9-DNMT3a. n = 3/group, * P < 0.05 vs. Scramble by Student’s t test. Images in A are representative from 3 replicate samples. ( C and D ) Macrophage migration assay ( C ) and inflammatory gene expression ( D ) in RAW264.7 macrophages cocultured with 3T3-L1 adipocytes infected with lentivirus expressing scramble sgRNA or S7 sgRNA targeting Esr1 promoter along with dCas9-TET1. n = 3/group, * P < 0.05 vs. Scramble by Student’s t test. Images in C are representative from 3 replicate samples. All data are expressed as mean ± SEM.

    Article Snippet: The mammalian lentiviral vectors FUW carrying dCas9-DNMT3a or dCas9-TET1, in which the catalytically inactive Cas9 (dCas9) has been engineered to be fused with the DNMT3a or TET1 ( , ), were purchased from Addgene (Addgene No. 84476 and 84475).

    Techniques: Migration, Gene Expression, Infection, Expressing

    ( A and B ) GTT ( A ) and ITT ( B ) in HFD-fed female C57BL/6J mice surgically injected with dCas9-TET1 along with either scramble sgRNA or S7 sgRNA targeting Esr1 promoter in gWAT, n = 4. * P < 0.05 vs. Scramble sgRNA by 1-way ANOVA with repeated measures followed by Fisher’s LSD post hoc test. ( C – E ) Gene expression ( C ), FACS analysis of F4/80 + CD11C + ATMs ( D ), and percentage of F4/80 + ATMs, F4/80 + CD11C + ATMs, and CD8 + T lymphocytes ( E ) in gWAT of HFD-fed female C57BL/6J mice surgically injected with dCas9-TET1 along with either scramble sgRNA or S7 sgRNA targeting Esr1 promoter in gWAT, n = 4. * P < 0.05 vs. Scramble sgRNA by Student’s t test. All data are expressed as mean ± SEM.

    Journal: JCI Insight

    Article Title: Epigenetic programming of estrogen receptor in adipocytes by high-fat diet regulates obesity-induced inflammation

    doi: 10.1172/jci.insight.173423

    Figure Lengend Snippet: ( A and B ) GTT ( A ) and ITT ( B ) in HFD-fed female C57BL/6J mice surgically injected with dCas9-TET1 along with either scramble sgRNA or S7 sgRNA targeting Esr1 promoter in gWAT, n = 4. * P < 0.05 vs. Scramble sgRNA by 1-way ANOVA with repeated measures followed by Fisher’s LSD post hoc test. ( C – E ) Gene expression ( C ), FACS analysis of F4/80 + CD11C + ATMs ( D ), and percentage of F4/80 + ATMs, F4/80 + CD11C + ATMs, and CD8 + T lymphocytes ( E ) in gWAT of HFD-fed female C57BL/6J mice surgically injected with dCas9-TET1 along with either scramble sgRNA or S7 sgRNA targeting Esr1 promoter in gWAT, n = 4. * P < 0.05 vs. Scramble sgRNA by Student’s t test. All data are expressed as mean ± SEM.

    Article Snippet: The mammalian lentiviral vectors FUW carrying dCas9-DNMT3a or dCas9-TET1, in which the catalytically inactive Cas9 (dCas9) has been engineered to be fused with the DNMT3a or TET1 ( , ), were purchased from Addgene (Addgene No. 84476 and 84475).

    Techniques: Injection, Gene Expression